2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. Figure 1. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. Compact designs. 20 μL of Component A was added to each well of the microplate. Learn more about its features, specifications, and applications in this user. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). SpectraMax Spectrofluorometers. Italic and boldface type are used for emphasis. 0 out of 5 $ 5,999. . Read one sample or up to 384 in a single plate read using any standard cuvette, or 96-. Using the. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. SpectraMax i3x Multi-Mode Detection Platform. . their consistent performance, reliability and durability. Microplate Readers. Microplate reader assays requiring infrared (IR) or red-shifted. on the SpectraMax iD3 microplate reader Introduction The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. Use the provided 3. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 470 nm). The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . 0200-6263. both cuvette-based and microplate reader applications on the same instrument. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. See SpectraMax® Microplate Reader User Manual Download Page. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). We use Spectramax M5 microplate reader in our lab. SpectraMax M5 reader settings are shown. This unlocks the transport slide. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. This is equivalent to having 511 built-in filters, The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. 000 Euro/Dollars for a high-end multi-mode microplate reader with several detection modes and the best technology for highest sensitivity and fastest detection. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. 313 reference gain check fail. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. In addition, the SpectraMax iD5Next generation SpectraMax® absorbance microplate readers. The powerful analysis tools in the software generate a standard curve and calculated data. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 33 Dramatic aggregation of Alzheimer A b by Cu(II) is induced by conditions representing physiological acidosis . Figure 1. 997 (n = 3). Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMinimum volume and maximum throughput with 96- or 384-well microplate compatibility. SAN JOSE, CA — Molecular Devices, LLC. 020 fmol 0. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. 230-1000 nm, Monochromator, ABS, FLR (top and bottom) and LUM, with incubation. Supplier: Molecular Devices. com. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. These lab workhorses are used in drug discovery. SpectraMax Mini User Guide. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Molecular Devices. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 2. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The 2-channel configuration is available with 0, 2, or 4 injectors. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Absorbance (optical density, OD) was read at 570 nm and 605. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Wide range of applications. Products. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). . 314 low lamp level warning. Bookings are free, but you will need to use iLab to book. Spectranet customer portalthe SpectraMax® M Series Multi-Mode Microplate Readers, for . Download PDF. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Microplate Spectrophotometers. Compact and tunable absorbance microplate readers DATA SHEET. SpectraMax Microplate Readers. It was also read on the monochromator-based. AlphaScreen. Page 28 Chapter 5: Read Modes and Read Types 1. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for. . Figure 3. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Detection modalities include absorbance (UV-Vis Abs) and. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Or scan up to 384 wells at any time to determine the best assay conditions. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. \ Figure 3. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Sensitive fluorescent quantitation of DNA with the Quant-iT. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. View the Molecular Devices website. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. Wide range of applications The VersaMax reader covers. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. both cuvette-based and microplate reader applications Microplate Readers. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Example: [Drawer]. 5 12. For SpectraMax ABS Plus reader, r 2 = 0. The Gemini EM can sample a variety of formats, from 6- to 384-well. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. microplate reader. A: Microplate Preparation. Step 1: Turn on your microplate reader. The chamber contains springs that position the cuvette in the proper alignment for a read. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. 304 signal level saturation. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. e. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. 1. The temperature in the plate chamber is ambient and isothermal. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. SpectraMax Plus: SpectraMax Plus. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalSpectraMax Microplate Readers. Example: [Drawer]. SoftMax® Pro 7. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. SpectraMax Spectrofluorometers. The plate reader optics are tunable for absorbance. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Other technologies such as imaging. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. 1. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Promega's ROS-Glo H202 assay can be combined with SpectraMax readers to explore the relationship between. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). It can run samples based on pre-defined. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Thank you!. Win7 Manual Spectramax-L or StakMax Driver Installation after Initial Install. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax Microplate Readers. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The cuvette must be in place when you read the plate. . Published Dec 16, 2020 | Updated May 19, 2023. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. keeping with best practices, reader performance should. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 303 unable to cal dark current. 0 out of 5 $ 2,999-20%. Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution: The SpectraMax® Mini Multi-Mode. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The advanced optical and. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. Endpoint, kinetic, spectrum. Fully tested with validations, warranty. 0 out of 5 $ 11,999. 2. . Temperature control. 306 plate air cal fail,low light. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. Results are shown in Table 1. Our SpectraTest® ABS2, FL1, LM1, SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Data exemplifying linear ranges from the three different kits are presented in Figure 2. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application. Devices’ StakMax® Microplate Stacker in a matter of minutes and . The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. Dual-Glo luciferase assay system (Promega Cat. SpectraMax L Microplate Reader This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. We would like to show you a description here but the site won’t allow us. Selection of Water Constant in PathCheck settings. Nucleic Acid and Protein Quantitation. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Please refer to the Obsolete Microplate Reader Models for more information. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. Or scan up to 384 wells at any time to determine the best assay conditions. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Molecular Devices SpectraMax M2E Microplate Reader. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Turn the reader off by pulling the power cord. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Download PDF. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The CellTiter-Glo assay from Promega uses luciferase. #6590-0131) Fluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. The analysis capabilities of the software provide quantitation in an easy-to-read, user customizable report format. The SpectraMax® ABS Plus Microplate Reader can run . Or, with the reader specifications noted from the Instrument Description section of your reader's. We would like to show you a description here but the site won’t allow us. The guide also covers the software features, the specifications, and the accessories of the systems. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. 313 reference gain check fail. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Blank subtraction and standard curve fitting were done automatically by the software. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. In . The screw remains inside the instrument. . Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Contact our support team with your questions regarding Molecular Devices products and applications. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Path Check Pathlength Measurement every Gemini EM Reader. Summary of Contents for Molecular Devices SpectraMax M2. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. The SpectraMax® ABS Plus Microplate Reader can run . Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. Cell Cytotoxicity assay procedure. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Users can install the ScanLater Western Blot Detection Cartridge in the SpectraMax Paradigm SystemMicroplate readers read biological, chemical or physical events that occur on a microtiter plate. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Maximum signal with lowest background. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Three low volume 24-well bottom slides. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. 305 reference level saturation. 303 unable to cal dark current. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. ATP standard curves read on SpectraMax microplate readers. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein. Detailed specifications of each model reader are reported in Section 7, “Specifications”. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. With optimized reagents, option to operate the. Cuvette Microplate wells. 1) Select the correct plate and the wells that you want to measure. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. The calculated LOQ values were 0. Microplate Readers BioTek Epoch 2 Microplate Reader. Open the top cover of the instrument. SpectraMax 250 User. Avoid losing high signal. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. Figure 1. 40266 Email: drugdiscoverytech@lifetech. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. Align the two slots on the bottle holder with the two pegs on the rail. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. These lab workhorses are used in drug discovery, research, bioassay. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. Microplate automation and detection instruments for walk away operation and high quality results. For readers without a cuvette port, simply check the box next to ‘PathCheck’. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. 76358-624. Detailed specifications of each model reader are reported in Section 7, “Specifications”. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. For readers without a cuvette port, simply check the box next to ‘PathCheck’. It also integrates with the. no. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. For more sample throughput, both readersThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. 0 out of 5 $ 23,995 $ 22,495. with SpectraMax Microplate Readers Introduction Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The eight-channel system consists of 8 sample beams and 8 reference beams, delivering both superior precision and speed of reading across the microplate. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. The drawing shows a standard microplate of 15 mm height covered with a lid. Positions H21-K22 contained plate blanks of PBS only. . 6% for the on-board optics (monochromators). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. If it looks fine, then proceed. , April 14, 2022 – Molecular Devices, LLC. The Gemini XPS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. The plate. 003 fmol 0. Absorbance (optical density, OD) was read at 570 nm and 605. 315 can't find zero order. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. 7°C and the bottom to 36. The SpectraMax® iD3 Multi Mode Microplate . The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Self Service Available. Lift up the lid over the chamber to insert or remove a cuvette. Here, the CVs for each plate were less than 3, and the difference between mean. It measures the optical density (OD) of samples in both 96- and 384-well. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. 2 only: SpectraMax Mini Update. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. An enhancer is provided in the kit where applicable to further reduce background fluorescence and to increase the. ELISA Plate Readers / Microplate Readers. For readers without a cuvette port, these options are not shown in the software. Compare plate readersFive-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Instrument settings used with the SpectraMax M5 Microplate Reader. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. Add to Cart. When the user applies a set temperature of 37°C, the top is heated to 37. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. For readers without a cuvette port, these options are not shown in the software.